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calcium calibration buffer kit 1 solutions  (Thermo Fisher)


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    Thermo Fisher calcium calibration buffer kit 1 solutions
    Calcium Calibration Buffer Kit 1 Solutions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calibration+buffer+solution+kit/10__1074_slash_jbc__m304193200-55-1-7
    Average 90 stars, based on 1 article reviews
    calcium calibration buffer kit 1 solutions - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Identifying Organic Chemicals with Acetylcholinesterase Inhibition in Nationwide Estuarine Waters by Machine Learning-Assisted Mass Spectrometric Screening
    Article Snippet: .. Quality assurance and quality control HRMS was calibrated by the Pierce ion calibration solution kit (Thermo Fisher Scientific Inc., Waltham, MA) every week to ensure mass accuracy < 5 ppm. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Comparing digital detection platforms in high sensitivity immune‐phenotyping of extracellular vesicles
    Article Snippet: .. EV samples and BSA standards for calibration curve were diluted in BCA solution (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, Rockford, USA) in 1:9 ratio and incubated for 30 min at 37°C. ..

    Incubation:

    Article Title: Comparing digital detection platforms in high sensitivity immune‐phenotyping of extracellular vesicles
    Article Snippet: .. EV samples and BSA standards for calibration curve were diluted in BCA solution (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, Rockford, USA) in 1:9 ratio and incubated for 30 min at 37°C. ..

    Mass Spectrometry:

    Article Title: Silicone Wristband Spray Ionization Mass Spectrometry for Combined Exposome and Metabolome Profiling
    Article Snippet: The target analytes were detected in full scan mode at m/z 50–1000 under positive and negative scan modes on the LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, San Jose, CA). .. The inlet temperature of the MS was set at 275 °C and the S-lens voltage to 55 V. The mass spectrometer was calibrated before each batch of measurements by using an ion mass calibration solution kit (Pierce positive/negative, Thermo Fisher, USA). .. Data analysis of the mass spectrum data was done in MATLAB.

    Article Title: Occurrence and Temporal Trends of Benzotriazole UV Stabilizers in Mollusks (2010-2018) from the Chinese Bohai Sea Revealed by Target, Suspect, and Nontarget Screening Analysis.
    Article Snippet: .. Mass spectrometer was tuned by the Pierce ion calibration solution kit (Thermo Fisher Scientific Inc., Waltham, MA) each week to ensure mass accuracy < 5 ppm throughout the instrumental analysis processes. ..

    Article Title: Identification of Hydroxylated Chlorinated Paraffins in Human Serum and Their Potential Metabolic Pathways.
    Article Snippet: Shortand medium-chain chlorinated paraffins (SCCPs and MCCPs) are frequently detected in humans.. However, information regarding their metabolites is still very limited.. Herein, target analysis and halogenationguided nontarget and suspect screening were conducted on serum samples using UHPLC-Orbitrap-HRMS.

    Article Title: Occurrence and Distribution of Antibacterial Quaternary Ammonium Compounds in Chinese Estuaries Revealed by Machine Learning-Assisted Mass Spectrometric Analysis
    Article Snippet: Quality assurance and quality control (QA/QC) 349 All glassware was soaked overnight in non-cationic detergent (Decon 90, Decon 350 Laboratories Limited) to avoid analyte adsorption on surface active sitesS11. .. Prior to 351 injection, the Orbitrap Fusion high-resolution mass spectrometer was tuned with the 352 Pierce ion calibration solution kit (Thermo Fisher Scientific Inc.) to ensure a mass 353 accuracy of less than 5 ppm. ..

    Amplex Red Cholesterol Assay:

    Article Title: Complementary gene regulation by NRF1 and NRF2 protects against hepatic cholesterol overload.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-4HNE Abcam Cat# ab46545; RRID: AB_722490 anti-F4/80 Cell signaling technologies Cat# 70076; RRID: AB_2799771 anti-GAPDH Invitrogen Cat# AM4300; RRID: AB_2536381 anti-LAMIN A/C Cell Signaling Technology Cat# #4777; RRID: AB_10545756 anti-mouse HRP conjugate Cell signaling technologies Cat# 7076S; RRID: AB_330924 anti-NRF1 Cell Signaling Technology Cat# 8052; RRID: AB_11178947 anti-NRF2 Cell Signaling Technology Cat# 12721; RRID: AB_10891429 anti-p62 Cell Signaling Technology Cat# 23214; RRID: AB_2798858 anti-rabbit HRP Conjugate Cell signaling technologies Cat# 7074S; RRID: AB_2099233 anti-YAP/TAZ Cell signaling technologies Cat# 8418; RRID: AB_10950494 Envision+ System-HRP Labeled Polymer Anti-Rabbit Dako Cat# K4003 Rabbit (DA1E) mAb IgG Cell Signaling Technology Cat #3900; RRID: AB_1550038 Bacterial and virus strains Adeno-associated virus expressing human NRF1(NM_003204.2) Pennsylvania Vector Biocore N/A Liver-targeting adeno-associated virus expressing Cre recombinase Pennsylvania Vector Biocore Addgene Cat# 107787-AAV8 Liver-targeting adeno-associated virus expressing GFP Pennsylvania Vector Biocore Addgene Cat# 105535-AAV8 Chemicals, peptides, and recombinant proteins Bardoxolone methyl Selleckchem Cat# S6647 ChIP-Grade Protein G Magnetic Beads Cell signaling technologies Cat# #9006 Diaminobenzidine(DAB) Dako Cat# K3468 DNAzol Invitrogen Cat# 10974020 Infinity Triglyceride Reagent ThermoFisher Scientific Cat# TR22421 Glass Slides VWR Cat # 48311-703 Mounting medium Polysciences Cat # 18606 Nitrocellulose membrane Bio-Rad Cat# 162-0115 NP-40 Alternative CalbiochemTM 492016100ML NuPAGE 4%–12% Bis-Tris Protein Gels ThermoFisher Scientific Cat# NP0336 NuPAGE MOPS Running Buffer ThermoFisher Scientific Cat# NP0001 PowerUpTM SYBRTM Green Master Mix ThermoFisher Scientific Cat# A25742 Scigen Tissue-PlusTM O.C.T. .. Compound Scigen Cat# 23-730-571 TRIzol ThermoFisher Scientific Cat# 15596018 Critical commercial assays Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Alanine Transaminase Colorimetric Activity Assay Kit Caymanchem Cat# 700260 GeneJET PCR Purification Kit ThermoFisher Scientific Cat# K0702 HDL and LDL/VLDL Quantitation Kit Sigma Cat# MAK045-1kt Maxima First Strand cDNA Synthesis Kit ThermoFisher Scientific Cat# K1641 Pierce LTQ Velos ESI Positive Ion Calibration Solution ThermoFisher Scientific Cat# 88322 (Continued on next page) Cell Reports 42, 112399, April 25, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Femto Maximum Sensitivity Substrate ThermoFisher Scientific Cat# 34096 TruSeq stranded mRNA library prep kit Illumina Cat# 20020594 TruSeq RNA UD Indexes Illumina Cat# 20022371 Deposited data RNA-seq and ChIPseq data This paper NCBI; BioProject ID: PRJNA806849 Proteomics data This paper ProteomeXchange: PXD032069 Unprocessed Data Files Mendeley data for this paper https://doi.org/10.17632/pw77wmhss9.1 Experimental models: Organisms/strains Mouse: C57bl6/J The Jackson Laboratory Jax: 000664 nfe2l1flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l2flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l1flox/flox; nfe2l2flox/flox Generated in house N/A Oligonucleotides See Table S6 for qPCR and ChIP-qPCR primer list This paper N/A Software and algorithms Aperio Imagescope viewing software (V.12) Leica Biosystems Imaging RRID:SCR_020993; https://www.leicabiosystems. com/ Bowtie2 Open-source software maintained byJohns Hopkins University RRID:SCR_016368; http://bowtie-bio.sourceforge. net/bowtie2/index.shtml DESeq2 Bioconductor RRID:SCR_015687; https://bioconductor.org/ packages/release/bioc/html/DESeq2.html Fiji open source image analysis software Fiji RRID:SCR_002285; https://fiji.sc/ GraphPad Prism 9.3.1 GraphPad Software RRID:SCR_002798; https://www.graphpad.com HOMER University of California San Diego RRID:SCR_010881; http://homer.ucsd.edu/ Illumina Generate FASTQ BaseSpace pipeline (V.1.1.0.64) Illumina RRID:SCR_01188; https://basespace.illumina. com/home/sequence MACS2 github RRID:SCR_013291; https://github.com/macs3- project/MACS MetaboAnalyst 4.0 R package MetaboAnalyst RRID:SCR_015539; https://www.metaboanalyst.ca/ Picard Broad Institute RRID:SCR_006525; http://broadinstitute.github.io/ picard/ Samtools Htslib RRID:SCR_002105; https://www.htslib.org Other Control diet (With 10 kcal% Fat) Research Diets Cat# D09100304; http://www.researchdiets.com/ High fat, fructose, and cholesterol (HFFC) diet Research Diets Cat# D19021910; http://www.researchdiets.com/ Regular chow Prolab RMH 3000 LabDiet Cat# 5058

    Activity Assay:

    Article Title: Complementary gene regulation by NRF1 and NRF2 protects against hepatic cholesterol overload.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-4HNE Abcam Cat# ab46545; RRID: AB_722490 anti-F4/80 Cell signaling technologies Cat# 70076; RRID: AB_2799771 anti-GAPDH Invitrogen Cat# AM4300; RRID: AB_2536381 anti-LAMIN A/C Cell Signaling Technology Cat# #4777; RRID: AB_10545756 anti-mouse HRP conjugate Cell signaling technologies Cat# 7076S; RRID: AB_330924 anti-NRF1 Cell Signaling Technology Cat# 8052; RRID: AB_11178947 anti-NRF2 Cell Signaling Technology Cat# 12721; RRID: AB_10891429 anti-p62 Cell Signaling Technology Cat# 23214; RRID: AB_2798858 anti-rabbit HRP Conjugate Cell signaling technologies Cat# 7074S; RRID: AB_2099233 anti-YAP/TAZ Cell signaling technologies Cat# 8418; RRID: AB_10950494 Envision+ System-HRP Labeled Polymer Anti-Rabbit Dako Cat# K4003 Rabbit (DA1E) mAb IgG Cell Signaling Technology Cat #3900; RRID: AB_1550038 Bacterial and virus strains Adeno-associated virus expressing human NRF1(NM_003204.2) Pennsylvania Vector Biocore N/A Liver-targeting adeno-associated virus expressing Cre recombinase Pennsylvania Vector Biocore Addgene Cat# 107787-AAV8 Liver-targeting adeno-associated virus expressing GFP Pennsylvania Vector Biocore Addgene Cat# 105535-AAV8 Chemicals, peptides, and recombinant proteins Bardoxolone methyl Selleckchem Cat# S6647 ChIP-Grade Protein G Magnetic Beads Cell signaling technologies Cat# #9006 Diaminobenzidine(DAB) Dako Cat# K3468 DNAzol Invitrogen Cat# 10974020 Infinity Triglyceride Reagent ThermoFisher Scientific Cat# TR22421 Glass Slides VWR Cat # 48311-703 Mounting medium Polysciences Cat # 18606 Nitrocellulose membrane Bio-Rad Cat# 162-0115 NP-40 Alternative CalbiochemTM 492016100ML NuPAGE 4%–12% Bis-Tris Protein Gels ThermoFisher Scientific Cat# NP0336 NuPAGE MOPS Running Buffer ThermoFisher Scientific Cat# NP0001 PowerUpTM SYBRTM Green Master Mix ThermoFisher Scientific Cat# A25742 Scigen Tissue-PlusTM O.C.T. .. Compound Scigen Cat# 23-730-571 TRIzol ThermoFisher Scientific Cat# 15596018 Critical commercial assays Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Alanine Transaminase Colorimetric Activity Assay Kit Caymanchem Cat# 700260 GeneJET PCR Purification Kit ThermoFisher Scientific Cat# K0702 HDL and LDL/VLDL Quantitation Kit Sigma Cat# MAK045-1kt Maxima First Strand cDNA Synthesis Kit ThermoFisher Scientific Cat# K1641 Pierce LTQ Velos ESI Positive Ion Calibration Solution ThermoFisher Scientific Cat# 88322 (Continued on next page) Cell Reports 42, 112399, April 25, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Femto Maximum Sensitivity Substrate ThermoFisher Scientific Cat# 34096 TruSeq stranded mRNA library prep kit Illumina Cat# 20020594 TruSeq RNA UD Indexes Illumina Cat# 20022371 Deposited data RNA-seq and ChIPseq data This paper NCBI; BioProject ID: PRJNA806849 Proteomics data This paper ProteomeXchange: PXD032069 Unprocessed Data Files Mendeley data for this paper https://doi.org/10.17632/pw77wmhss9.1 Experimental models: Organisms/strains Mouse: C57bl6/J The Jackson Laboratory Jax: 000664 nfe2l1flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l2flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l1flox/flox; nfe2l2flox/flox Generated in house N/A Oligonucleotides See Table S6 for qPCR and ChIP-qPCR primer list This paper N/A Software and algorithms Aperio Imagescope viewing software (V.12) Leica Biosystems Imaging RRID:SCR_020993; https://www.leicabiosystems. com/ Bowtie2 Open-source software maintained byJohns Hopkins University RRID:SCR_016368; http://bowtie-bio.sourceforge. net/bowtie2/index.shtml DESeq2 Bioconductor RRID:SCR_015687; https://bioconductor.org/ packages/release/bioc/html/DESeq2.html Fiji open source image analysis software Fiji RRID:SCR_002285; https://fiji.sc/ GraphPad Prism 9.3.1 GraphPad Software RRID:SCR_002798; https://www.graphpad.com HOMER University of California San Diego RRID:SCR_010881; http://homer.ucsd.edu/ Illumina Generate FASTQ BaseSpace pipeline (V.1.1.0.64) Illumina RRID:SCR_01188; https://basespace.illumina. com/home/sequence MACS2 github RRID:SCR_013291; https://github.com/macs3- project/MACS MetaboAnalyst 4.0 R package MetaboAnalyst RRID:SCR_015539; https://www.metaboanalyst.ca/ Picard Broad Institute RRID:SCR_006525; http://broadinstitute.github.io/ picard/ Samtools Htslib RRID:SCR_002105; https://www.htslib.org Other Control diet (With 10 kcal% Fat) Research Diets Cat# D09100304; http://www.researchdiets.com/ High fat, fructose, and cholesterol (HFFC) diet Research Diets Cat# D19021910; http://www.researchdiets.com/ Regular chow Prolab RMH 3000 LabDiet Cat# 5058

    Polymerase Chain Reaction:

    Article Title: Complementary gene regulation by NRF1 and NRF2 protects against hepatic cholesterol overload.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-4HNE Abcam Cat# ab46545; RRID: AB_722490 anti-F4/80 Cell signaling technologies Cat# 70076; RRID: AB_2799771 anti-GAPDH Invitrogen Cat# AM4300; RRID: AB_2536381 anti-LAMIN A/C Cell Signaling Technology Cat# #4777; RRID: AB_10545756 anti-mouse HRP conjugate Cell signaling technologies Cat# 7076S; RRID: AB_330924 anti-NRF1 Cell Signaling Technology Cat# 8052; RRID: AB_11178947 anti-NRF2 Cell Signaling Technology Cat# 12721; RRID: AB_10891429 anti-p62 Cell Signaling Technology Cat# 23214; RRID: AB_2798858 anti-rabbit HRP Conjugate Cell signaling technologies Cat# 7074S; RRID: AB_2099233 anti-YAP/TAZ Cell signaling technologies Cat# 8418; RRID: AB_10950494 Envision+ System-HRP Labeled Polymer Anti-Rabbit Dako Cat# K4003 Rabbit (DA1E) mAb IgG Cell Signaling Technology Cat #3900; RRID: AB_1550038 Bacterial and virus strains Adeno-associated virus expressing human NRF1(NM_003204.2) Pennsylvania Vector Biocore N/A Liver-targeting adeno-associated virus expressing Cre recombinase Pennsylvania Vector Biocore Addgene Cat# 107787-AAV8 Liver-targeting adeno-associated virus expressing GFP Pennsylvania Vector Biocore Addgene Cat# 105535-AAV8 Chemicals, peptides, and recombinant proteins Bardoxolone methyl Selleckchem Cat# S6647 ChIP-Grade Protein G Magnetic Beads Cell signaling technologies Cat# #9006 Diaminobenzidine(DAB) Dako Cat# K3468 DNAzol Invitrogen Cat# 10974020 Infinity Triglyceride Reagent ThermoFisher Scientific Cat# TR22421 Glass Slides VWR Cat # 48311-703 Mounting medium Polysciences Cat # 18606 Nitrocellulose membrane Bio-Rad Cat# 162-0115 NP-40 Alternative CalbiochemTM 492016100ML NuPAGE 4%–12% Bis-Tris Protein Gels ThermoFisher Scientific Cat# NP0336 NuPAGE MOPS Running Buffer ThermoFisher Scientific Cat# NP0001 PowerUpTM SYBRTM Green Master Mix ThermoFisher Scientific Cat# A25742 Scigen Tissue-PlusTM O.C.T. .. Compound Scigen Cat# 23-730-571 TRIzol ThermoFisher Scientific Cat# 15596018 Critical commercial assays Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Alanine Transaminase Colorimetric Activity Assay Kit Caymanchem Cat# 700260 GeneJET PCR Purification Kit ThermoFisher Scientific Cat# K0702 HDL and LDL/VLDL Quantitation Kit Sigma Cat# MAK045-1kt Maxima First Strand cDNA Synthesis Kit ThermoFisher Scientific Cat# K1641 Pierce LTQ Velos ESI Positive Ion Calibration Solution ThermoFisher Scientific Cat# 88322 (Continued on next page) Cell Reports 42, 112399, April 25, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Femto Maximum Sensitivity Substrate ThermoFisher Scientific Cat# 34096 TruSeq stranded mRNA library prep kit Illumina Cat# 20020594 TruSeq RNA UD Indexes Illumina Cat# 20022371 Deposited data RNA-seq and ChIPseq data This paper NCBI; BioProject ID: PRJNA806849 Proteomics data This paper ProteomeXchange: PXD032069 Unprocessed Data Files Mendeley data for this paper https://doi.org/10.17632/pw77wmhss9.1 Experimental models: Organisms/strains Mouse: C57bl6/J The Jackson Laboratory Jax: 000664 nfe2l1flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l2flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l1flox/flox; nfe2l2flox/flox Generated in house N/A Oligonucleotides See Table S6 for qPCR and ChIP-qPCR primer list This paper N/A Software and algorithms Aperio Imagescope viewing software (V.12) Leica Biosystems Imaging RRID:SCR_020993; https://www.leicabiosystems. com/ Bowtie2 Open-source software maintained byJohns Hopkins University RRID:SCR_016368; http://bowtie-bio.sourceforge. net/bowtie2/index.shtml DESeq2 Bioconductor RRID:SCR_015687; https://bioconductor.org/ packages/release/bioc/html/DESeq2.html Fiji open source image analysis software Fiji RRID:SCR_002285; https://fiji.sc/ GraphPad Prism 9.3.1 GraphPad Software RRID:SCR_002798; https://www.graphpad.com HOMER University of California San Diego RRID:SCR_010881; http://homer.ucsd.edu/ Illumina Generate FASTQ BaseSpace pipeline (V.1.1.0.64) Illumina RRID:SCR_01188; https://basespace.illumina. com/home/sequence MACS2 github RRID:SCR_013291; https://github.com/macs3- project/MACS MetaboAnalyst 4.0 R package MetaboAnalyst RRID:SCR_015539; https://www.metaboanalyst.ca/ Picard Broad Institute RRID:SCR_006525; http://broadinstitute.github.io/ picard/ Samtools Htslib RRID:SCR_002105; https://www.htslib.org Other Control diet (With 10 kcal% Fat) Research Diets Cat# D09100304; http://www.researchdiets.com/ High fat, fructose, and cholesterol (HFFC) diet Research Diets Cat# D19021910; http://www.researchdiets.com/ Regular chow Prolab RMH 3000 LabDiet Cat# 5058

    Purification:

    Article Title: Complementary gene regulation by NRF1 and NRF2 protects against hepatic cholesterol overload.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-4HNE Abcam Cat# ab46545; RRID: AB_722490 anti-F4/80 Cell signaling technologies Cat# 70076; RRID: AB_2799771 anti-GAPDH Invitrogen Cat# AM4300; RRID: AB_2536381 anti-LAMIN A/C Cell Signaling Technology Cat# #4777; RRID: AB_10545756 anti-mouse HRP conjugate Cell signaling technologies Cat# 7076S; RRID: AB_330924 anti-NRF1 Cell Signaling Technology Cat# 8052; RRID: AB_11178947 anti-NRF2 Cell Signaling Technology Cat# 12721; RRID: AB_10891429 anti-p62 Cell Signaling Technology Cat# 23214; RRID: AB_2798858 anti-rabbit HRP Conjugate Cell signaling technologies Cat# 7074S; RRID: AB_2099233 anti-YAP/TAZ Cell signaling technologies Cat# 8418; RRID: AB_10950494 Envision+ System-HRP Labeled Polymer Anti-Rabbit Dako Cat# K4003 Rabbit (DA1E) mAb IgG Cell Signaling Technology Cat #3900; RRID: AB_1550038 Bacterial and virus strains Adeno-associated virus expressing human NRF1(NM_003204.2) Pennsylvania Vector Biocore N/A Liver-targeting adeno-associated virus expressing Cre recombinase Pennsylvania Vector Biocore Addgene Cat# 107787-AAV8 Liver-targeting adeno-associated virus expressing GFP Pennsylvania Vector Biocore Addgene Cat# 105535-AAV8 Chemicals, peptides, and recombinant proteins Bardoxolone methyl Selleckchem Cat# S6647 ChIP-Grade Protein G Magnetic Beads Cell signaling technologies Cat# #9006 Diaminobenzidine(DAB) Dako Cat# K3468 DNAzol Invitrogen Cat# 10974020 Infinity Triglyceride Reagent ThermoFisher Scientific Cat# TR22421 Glass Slides VWR Cat # 48311-703 Mounting medium Polysciences Cat # 18606 Nitrocellulose membrane Bio-Rad Cat# 162-0115 NP-40 Alternative CalbiochemTM 492016100ML NuPAGE 4%–12% Bis-Tris Protein Gels ThermoFisher Scientific Cat# NP0336 NuPAGE MOPS Running Buffer ThermoFisher Scientific Cat# NP0001 PowerUpTM SYBRTM Green Master Mix ThermoFisher Scientific Cat# A25742 Scigen Tissue-PlusTM O.C.T. .. Compound Scigen Cat# 23-730-571 TRIzol ThermoFisher Scientific Cat# 15596018 Critical commercial assays Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Alanine Transaminase Colorimetric Activity Assay Kit Caymanchem Cat# 700260 GeneJET PCR Purification Kit ThermoFisher Scientific Cat# K0702 HDL and LDL/VLDL Quantitation Kit Sigma Cat# MAK045-1kt Maxima First Strand cDNA Synthesis Kit ThermoFisher Scientific Cat# K1641 Pierce LTQ Velos ESI Positive Ion Calibration Solution ThermoFisher Scientific Cat# 88322 (Continued on next page) Cell Reports 42, 112399, April 25, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Femto Maximum Sensitivity Substrate ThermoFisher Scientific Cat# 34096 TruSeq stranded mRNA library prep kit Illumina Cat# 20020594 TruSeq RNA UD Indexes Illumina Cat# 20022371 Deposited data RNA-seq and ChIPseq data This paper NCBI; BioProject ID: PRJNA806849 Proteomics data This paper ProteomeXchange: PXD032069 Unprocessed Data Files Mendeley data for this paper https://doi.org/10.17632/pw77wmhss9.1 Experimental models: Organisms/strains Mouse: C57bl6/J The Jackson Laboratory Jax: 000664 nfe2l1flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l2flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l1flox/flox; nfe2l2flox/flox Generated in house N/A Oligonucleotides See Table S6 for qPCR and ChIP-qPCR primer list This paper N/A Software and algorithms Aperio Imagescope viewing software (V.12) Leica Biosystems Imaging RRID:SCR_020993; https://www.leicabiosystems. com/ Bowtie2 Open-source software maintained byJohns Hopkins University RRID:SCR_016368; http://bowtie-bio.sourceforge. net/bowtie2/index.shtml DESeq2 Bioconductor RRID:SCR_015687; https://bioconductor.org/ packages/release/bioc/html/DESeq2.html Fiji open source image analysis software Fiji RRID:SCR_002285; https://fiji.sc/ GraphPad Prism 9.3.1 GraphPad Software RRID:SCR_002798; https://www.graphpad.com HOMER University of California San Diego RRID:SCR_010881; http://homer.ucsd.edu/ Illumina Generate FASTQ BaseSpace pipeline (V.1.1.0.64) Illumina RRID:SCR_01188; https://basespace.illumina. com/home/sequence MACS2 github RRID:SCR_013291; https://github.com/macs3- project/MACS MetaboAnalyst 4.0 R package MetaboAnalyst RRID:SCR_015539; https://www.metaboanalyst.ca/ Picard Broad Institute RRID:SCR_006525; http://broadinstitute.github.io/ picard/ Samtools Htslib RRID:SCR_002105; https://www.htslib.org Other Control diet (With 10 kcal% Fat) Research Diets Cat# D09100304; http://www.researchdiets.com/ High fat, fructose, and cholesterol (HFFC) diet Research Diets Cat# D19021910; http://www.researchdiets.com/ Regular chow Prolab RMH 3000 LabDiet Cat# 5058

    Quantitation Assay:

    Article Title: Complementary gene regulation by NRF1 and NRF2 protects against hepatic cholesterol overload.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-4HNE Abcam Cat# ab46545; RRID: AB_722490 anti-F4/80 Cell signaling technologies Cat# 70076; RRID: AB_2799771 anti-GAPDH Invitrogen Cat# AM4300; RRID: AB_2536381 anti-LAMIN A/C Cell Signaling Technology Cat# #4777; RRID: AB_10545756 anti-mouse HRP conjugate Cell signaling technologies Cat# 7076S; RRID: AB_330924 anti-NRF1 Cell Signaling Technology Cat# 8052; RRID: AB_11178947 anti-NRF2 Cell Signaling Technology Cat# 12721; RRID: AB_10891429 anti-p62 Cell Signaling Technology Cat# 23214; RRID: AB_2798858 anti-rabbit HRP Conjugate Cell signaling technologies Cat# 7074S; RRID: AB_2099233 anti-YAP/TAZ Cell signaling technologies Cat# 8418; RRID: AB_10950494 Envision+ System-HRP Labeled Polymer Anti-Rabbit Dako Cat# K4003 Rabbit (DA1E) mAb IgG Cell Signaling Technology Cat #3900; RRID: AB_1550038 Bacterial and virus strains Adeno-associated virus expressing human NRF1(NM_003204.2) Pennsylvania Vector Biocore N/A Liver-targeting adeno-associated virus expressing Cre recombinase Pennsylvania Vector Biocore Addgene Cat# 107787-AAV8 Liver-targeting adeno-associated virus expressing GFP Pennsylvania Vector Biocore Addgene Cat# 105535-AAV8 Chemicals, peptides, and recombinant proteins Bardoxolone methyl Selleckchem Cat# S6647 ChIP-Grade Protein G Magnetic Beads Cell signaling technologies Cat# #9006 Diaminobenzidine(DAB) Dako Cat# K3468 DNAzol Invitrogen Cat# 10974020 Infinity Triglyceride Reagent ThermoFisher Scientific Cat# TR22421 Glass Slides VWR Cat # 48311-703 Mounting medium Polysciences Cat # 18606 Nitrocellulose membrane Bio-Rad Cat# 162-0115 NP-40 Alternative CalbiochemTM 492016100ML NuPAGE 4%–12% Bis-Tris Protein Gels ThermoFisher Scientific Cat# NP0336 NuPAGE MOPS Running Buffer ThermoFisher Scientific Cat# NP0001 PowerUpTM SYBRTM Green Master Mix ThermoFisher Scientific Cat# A25742 Scigen Tissue-PlusTM O.C.T. .. Compound Scigen Cat# 23-730-571 TRIzol ThermoFisher Scientific Cat# 15596018 Critical commercial assays Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Alanine Transaminase Colorimetric Activity Assay Kit Caymanchem Cat# 700260 GeneJET PCR Purification Kit ThermoFisher Scientific Cat# K0702 HDL and LDL/VLDL Quantitation Kit Sigma Cat# MAK045-1kt Maxima First Strand cDNA Synthesis Kit ThermoFisher Scientific Cat# K1641 Pierce LTQ Velos ESI Positive Ion Calibration Solution ThermoFisher Scientific Cat# 88322 (Continued on next page) Cell Reports 42, 112399, April 25, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Femto Maximum Sensitivity Substrate ThermoFisher Scientific Cat# 34096 TruSeq stranded mRNA library prep kit Illumina Cat# 20020594 TruSeq RNA UD Indexes Illumina Cat# 20022371 Deposited data RNA-seq and ChIPseq data This paper NCBI; BioProject ID: PRJNA806849 Proteomics data This paper ProteomeXchange: PXD032069 Unprocessed Data Files Mendeley data for this paper https://doi.org/10.17632/pw77wmhss9.1 Experimental models: Organisms/strains Mouse: C57bl6/J The Jackson Laboratory Jax: 000664 nfe2l1flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l2flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l1flox/flox; nfe2l2flox/flox Generated in house N/A Oligonucleotides See Table S6 for qPCR and ChIP-qPCR primer list This paper N/A Software and algorithms Aperio Imagescope viewing software (V.12) Leica Biosystems Imaging RRID:SCR_020993; https://www.leicabiosystems. com/ Bowtie2 Open-source software maintained byJohns Hopkins University RRID:SCR_016368; http://bowtie-bio.sourceforge. net/bowtie2/index.shtml DESeq2 Bioconductor RRID:SCR_015687; https://bioconductor.org/ packages/release/bioc/html/DESeq2.html Fiji open source image analysis software Fiji RRID:SCR_002285; https://fiji.sc/ GraphPad Prism 9.3.1 GraphPad Software RRID:SCR_002798; https://www.graphpad.com HOMER University of California San Diego RRID:SCR_010881; http://homer.ucsd.edu/ Illumina Generate FASTQ BaseSpace pipeline (V.1.1.0.64) Illumina RRID:SCR_01188; https://basespace.illumina. com/home/sequence MACS2 github RRID:SCR_013291; https://github.com/macs3- project/MACS MetaboAnalyst 4.0 R package MetaboAnalyst RRID:SCR_015539; https://www.metaboanalyst.ca/ Picard Broad Institute RRID:SCR_006525; http://broadinstitute.github.io/ picard/ Samtools Htslib RRID:SCR_002105; https://www.htslib.org Other Control diet (With 10 kcal% Fat) Research Diets Cat# D09100304; http://www.researchdiets.com/ High fat, fructose, and cholesterol (HFFC) diet Research Diets Cat# D19021910; http://www.researchdiets.com/ Regular chow Prolab RMH 3000 LabDiet Cat# 5058

    cDNA Synthesis:

    Article Title: Complementary gene regulation by NRF1 and NRF2 protects against hepatic cholesterol overload.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-4HNE Abcam Cat# ab46545; RRID: AB_722490 anti-F4/80 Cell signaling technologies Cat# 70076; RRID: AB_2799771 anti-GAPDH Invitrogen Cat# AM4300; RRID: AB_2536381 anti-LAMIN A/C Cell Signaling Technology Cat# #4777; RRID: AB_10545756 anti-mouse HRP conjugate Cell signaling technologies Cat# 7076S; RRID: AB_330924 anti-NRF1 Cell Signaling Technology Cat# 8052; RRID: AB_11178947 anti-NRF2 Cell Signaling Technology Cat# 12721; RRID: AB_10891429 anti-p62 Cell Signaling Technology Cat# 23214; RRID: AB_2798858 anti-rabbit HRP Conjugate Cell signaling technologies Cat# 7074S; RRID: AB_2099233 anti-YAP/TAZ Cell signaling technologies Cat# 8418; RRID: AB_10950494 Envision+ System-HRP Labeled Polymer Anti-Rabbit Dako Cat# K4003 Rabbit (DA1E) mAb IgG Cell Signaling Technology Cat #3900; RRID: AB_1550038 Bacterial and virus strains Adeno-associated virus expressing human NRF1(NM_003204.2) Pennsylvania Vector Biocore N/A Liver-targeting adeno-associated virus expressing Cre recombinase Pennsylvania Vector Biocore Addgene Cat# 107787-AAV8 Liver-targeting adeno-associated virus expressing GFP Pennsylvania Vector Biocore Addgene Cat# 105535-AAV8 Chemicals, peptides, and recombinant proteins Bardoxolone methyl Selleckchem Cat# S6647 ChIP-Grade Protein G Magnetic Beads Cell signaling technologies Cat# #9006 Diaminobenzidine(DAB) Dako Cat# K3468 DNAzol Invitrogen Cat# 10974020 Infinity Triglyceride Reagent ThermoFisher Scientific Cat# TR22421 Glass Slides VWR Cat # 48311-703 Mounting medium Polysciences Cat # 18606 Nitrocellulose membrane Bio-Rad Cat# 162-0115 NP-40 Alternative CalbiochemTM 492016100ML NuPAGE 4%–12% Bis-Tris Protein Gels ThermoFisher Scientific Cat# NP0336 NuPAGE MOPS Running Buffer ThermoFisher Scientific Cat# NP0001 PowerUpTM SYBRTM Green Master Mix ThermoFisher Scientific Cat# A25742 Scigen Tissue-PlusTM O.C.T. .. Compound Scigen Cat# 23-730-571 TRIzol ThermoFisher Scientific Cat# 15596018 Critical commercial assays Amplex Red Cholesterol Assay Kit Invitrogen Cat# A12216 Alanine Transaminase Colorimetric Activity Assay Kit Caymanchem Cat# 700260 GeneJET PCR Purification Kit ThermoFisher Scientific Cat# K0702 HDL and LDL/VLDL Quantitation Kit Sigma Cat# MAK045-1kt Maxima First Strand cDNA Synthesis Kit ThermoFisher Scientific Cat# K1641 Pierce LTQ Velos ESI Positive Ion Calibration Solution ThermoFisher Scientific Cat# 88322 (Continued on next page) Cell Reports 42, 112399, April 25, 2023 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Super Signal West Femto Maximum Sensitivity Substrate ThermoFisher Scientific Cat# 34096 TruSeq stranded mRNA library prep kit Illumina Cat# 20020594 TruSeq RNA UD Indexes Illumina Cat# 20022371 Deposited data RNA-seq and ChIPseq data This paper NCBI; BioProject ID: PRJNA806849 Proteomics data This paper ProteomeXchange: PXD032069 Unprocessed Data Files Mendeley data for this paper https://doi.org/10.17632/pw77wmhss9.1 Experimental models: Organisms/strains Mouse: C57bl6/J The Jackson Laboratory Jax: 000664 nfe2l1flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l2flox/flox A gift from G. Hotamisligil Widenmaier et al.35 nfe2l1flox/flox; nfe2l2flox/flox Generated in house N/A Oligonucleotides See Table S6 for qPCR and ChIP-qPCR primer list This paper N/A Software and algorithms Aperio Imagescope viewing software (V.12) Leica Biosystems Imaging RRID:SCR_020993; https://www.leicabiosystems. com/ Bowtie2 Open-source software maintained byJohns Hopkins University RRID:SCR_016368; http://bowtie-bio.sourceforge. net/bowtie2/index.shtml DESeq2 Bioconductor RRID:SCR_015687; https://bioconductor.org/ packages/release/bioc/html/DESeq2.html Fiji open source image analysis software Fiji RRID:SCR_002285; https://fiji.sc/ GraphPad Prism 9.3.1 GraphPad Software RRID:SCR_002798; https://www.graphpad.com HOMER University of California San Diego RRID:SCR_010881; http://homer.ucsd.edu/ Illumina Generate FASTQ BaseSpace pipeline (V.1.1.0.64) Illumina RRID:SCR_01188; https://basespace.illumina. com/home/sequence MACS2 github RRID:SCR_013291; https://github.com/macs3- project/MACS MetaboAnalyst 4.0 R package MetaboAnalyst RRID:SCR_015539; https://www.metaboanalyst.ca/ Picard Broad Institute RRID:SCR_006525; http://broadinstitute.github.io/ picard/ Samtools Htslib RRID:SCR_002105; https://www.htslib.org Other Control diet (With 10 kcal% Fat) Research Diets Cat# D09100304; http://www.researchdiets.com/ High fat, fructose, and cholesterol (HFFC) diet Research Diets Cat# D19021910; http://www.researchdiets.com/ Regular chow Prolab RMH 3000 LabDiet Cat# 5058

    Injection:

    Article Title: Occurrence and Distribution of Antibacterial Quaternary Ammonium Compounds in Chinese Estuaries Revealed by Machine Learning-Assisted Mass Spectrometric Analysis
    Article Snippet: Quality assurance and quality control (QA/QC) 349 All glassware was soaked overnight in non-cationic detergent (Decon 90, Decon 350 Laboratories Limited) to avoid analyte adsorption on surface active sitesS11. .. Prior to 351 injection, the Orbitrap Fusion high-resolution mass spectrometer was tuned with the 352 Pierce ion calibration solution kit (Thermo Fisher Scientific Inc.) to ensure a mass 353 accuracy of less than 5 ppm. ..



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